Deleted
Sample Discontinued
Experience: None
Not Used
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Coding
FadL

Part:BBa_K861000:Design

Designed by: Jiawei Huang   Group: iGEM12_WHU-China   (2012-08-15)


FadL coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1012
    Illegal EcoRI site found at 90
    Illegal XbaI site found at 97
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 90
    Illegal SpeI site found at 1013
    Illegal PstI site found at 1027
    Illegal NotI site found at 1020
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 90
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1013
    Illegal EcoRI site found at 90
    Illegal XbaI site found at 97
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 90
    Illegal XbaI site found at 97
    Illegal SpeI site found at 1013
    Illegal PstI site found at 1027
    Illegal NgoMIV site found at 172
    Illegal AgeI site found at 490
    Illegal AgeI site found at 613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 886


Design Notes

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Source

e.coli DH5a

References